Effect of Cadmium and Vitamin C on the Growth and Vitamin Contents of Citrobacter Freundii

Citrobacter freundii bacteria were grown in LB medium containing cadmium ion. In order to reduce adverse effect caused by cadmium, different concentrations of vitamin C, added the growth medium. While concentration was determined spectrophotometer, amounts water and fat-soluble vitamins also HPLC. The varied from 0 (control), 10, 20, 40, 60, 75, 100, 125 ppm has not been observed on bacteria, up 40 ppm, other hand no significant microorganism over 150 cadmium. Significant change 100 Cd. Microorganism decreased 18.8, 40.5 45.5 percent Cadmium respectively. With addition 75 C same medium, about 2.0, 28.0 31.0 percent, respectively compared control. addition, fat water-soluble cadmium-containing comparison control (p<0.05). 25, 50 soluble increased. As a result, it can be said that negative effects bacterial content reversed bacteria.